agilent fld manual
This manual provides comprehensive guidance for the Agilent 1260 Infinity II FLD, covering installation, operation, and maintenance. It ensures optimal performance and compliance with safety standards.
1.1. Overview of the Agilent 1260 Infinity II FLD
The Agilent 1260 Infinity II FLD is a high-sensitivity fluorescence detector designed for laboratory applications, offering exceptional selectivity and reliability. It features a user-friendly interface and advanced optical design, ensuring precise detection of fluorescent compounds. The detector is part of the Agilent InfinityLab series, known for its cutting-edge technology and versatility. Key components include an 8 µl flow cell and a LAN interface for seamless integration with laboratory systems. Optional accessories, such as a flow-through cuvette, expand its functionality for spectrofluorometric measurements. This detector is ideal for trace-level analysis in various fields, including pharmaceutical, environmental, and biotechnological research. Its robust design and high performance make it a trusted tool for demanding laboratory workflows.
1.2. Importance of the FLD in Laboratory Applications
The Agilent 1260 Infinity II FLD plays a critical role in laboratory settings due to its high sensitivity and selectivity, enabling precise detection of fluorescent compounds at trace levels. Its ability to provide accurate and reliable results makes it indispensable in various analytical workflows. The detector is widely used in pharmaceutical, biotechnological, and environmental research for applications such as impurity detection, protein analysis, and the quantification of fluorescent-labeled compounds. Its high performance ensures compliance with stringent regulatory standards, while its versatility supports a broad range of experimental demands. By delivering consistent and reproducible data, the FLD contributes significantly to advancing scientific discovery and maintaining high-quality laboratory outcomes.
1.3. Structure and Content of the Manual
This manual is organized into eight main sections, each addressing specific aspects of the Agilent 1260 Infinity II FLD. The introduction provides an overview of the detector, its importance, and the manual’s structure. Subsequent sections detail product description, operation principles, installation, maintenance, safety information, performance, and appendices. The appendices include a glossary, contact information, and additional resources. The manual is designed to guide users through installation, operation, troubleshooting, and maintenance, ensuring optimal performance and compliance with safety standards. Each section is structured to provide clear, concise information, making it easy for users to navigate and find relevant details efficiently.
Product Description and Specifications
The Agilent 1260 Infinity II FLD offers high-sensitivity fluorescence detection with an 8 µl flow cell and LAN interface. Optional accessories include a flow-through cuvette (G1321B).
2.1. Key Features of the Agilent 1260 Infinity II FLD
The Agilent 1260 Infinity II FLD is designed for high-sensitivity fluorescence detection, with features like an 8 µl flow cell and a LAN interface for seamless connectivity. It includes advanced software integration for data acquisition and analysis, ensuring precise control over detection parameters. The detector also offers flexibility with optional accessories, such as a flow-through cuvette, enabling it to function as a fluorescence spectrometer. Its robust design and user-friendly interface make it suitable for various laboratory applications, including trace-level component analysis. The FLD supports both standard and custom methods, catering to diverse analytical needs while maintaining high performance and reliability.
2.2. Technical Specifications of the Detector
The Agilent 1260 Infinity II FLD features a high-sensitivity fluorescence detection system with an 8 µl flow cell and LAN interface for advanced connectivity. The emission monochromator operates within a range of 200 nm to 1200 nm, ensuring precise wavelength selection. The detector supports data rates up to 74 Hz, making it suitable for high-speed chromatography. It includes a flow-through cuvette option for use as a fluorescence spectrometer. The system is compatible with Agilent’s software suite for data acquisition and analysis. Optional configurations, such as deleting the LAN card, are available for specialized applications. The detector’s design ensures low noise and high linearity, with a limit of detection below 100 fmol for most compounds.
2.3. Optional Accessories and Upgrades
The Agilent 1260 Infinity II FLD offers optional accessories to enhance functionality. The G1321B flow-through cuvette enables the detector to function as a fluorescence spectrometer, ideal for offline measurements. An optional LAN card deletion (option 025) is available for systems not requiring network connectivity. Additional upgrades include replacement interface boards for improved compatibility and flow cells of varying path lengths to suit specific analytical needs. Maintenance kits, such as spare lamps and seals, ensure prolonged instrument lifespan. Software upgrades can also be installed to access advanced features like multi-signal detection and spectral analysis. These optional upgrades allow users to tailor the detector to their specific laboratory requirements, optimizing performance and versatility for diverse applications.
2.4. Ordering Details and Product Numbers
When ordering the Agilent 1260 Infinity II FLD, refer to product number G1321B for the base model, which includes an 8 µl flow cell and LAN interface with cable. To customize your system, use option 025 to delete the LAN card if network connectivity is not required. Optional accessories like the flow-through cuvette for spectrometer functionality are available under specific part numbers. Ensure to verify all product numbers and options with Agilent Technologies or authorized distributors to ensure compatibility and meet your laboratory needs effectively. Proper ordering ensures seamless integration and optimal performance of the detector in your chromatography setup.
How the Detector Operates
The Agilent 1260 Infinity II FLD operates by exciting molecules with light, causing them to emit fluorescence. The detector measures this emitted light to identify and quantify analytes.
3.1. Principles of Fluorescence Detection
Fluorescence detection relies on the ability of certain molecules to emit light after absorbing excitation energy. When a sample is illuminated with light at a specific wavelength, the molecules absorb it, exciting their electrons. As these electrons return to their ground state, they emit light at a longer wavelength. The Agilent 1260 Infinity II FLD measures this emitted light, allowing for the detection and quantification of fluorescent compounds. The detector’s sensitivity and selectivity are enhanced by optimizing excitation and emission wavelengths for specific analytes. This principle enables high-performance analysis in various applications, including trace-level component detection, making it a powerful tool in modern laboratory settings.
3.2. Luminescence Detection and Excitation Processes
Luminescence detection in the Agilent 1260 Infinity II FLD involves the emission of light by molecules transitioning from an excited state to their ground state. Excitation occurs when molecules absorb light at a specific wavelength, provided by the detector’s light source. The excitation monochromator filters this light to the desired wavelength, ensuring optimal energy transfer. The sample, passing through the flow cell, emits light at a longer wavelength, which is then measured by the detector. This process is highly sensitive and selective, allowing for precise detection of fluorescent compounds. The detector’s design, including the emission monochromator, enhances signal-to-noise ratio, making it ideal for trace-level analysis in various laboratory applications.
3.3. Types of Fluorescence Detection (FLD vs. DAD)
The Agilent 1260 Infinity II FLD and Diode Array Detector (DAD) serve distinct roles in fluorescence and absorbance detection. FLD excels in sensitivity and selectivity for fluorescent compounds, ideal for trace-level analysis. DAD, however, captures a broad spectrum of absorbance data, offering versatility for various analytes. While FLD targets specific emission wavelengths, DAD provides comprehensive UV-Vis data, making it suitable for general chromatography. Together, they complement each other in advanced analytical workflows, ensuring precise detection for diverse applications.
Installation and Setup
Unpack and prepare the Agilent 1260 Infinity II FLD, ensuring proper flow cell installation and connections. Follow software setup requirements for optimal system integration and performance.
4.1. Unpacking and Preparing the Detector
Unpacking the Agilent 1260 Infinity II FLD requires careful handling to avoid damage. Inspect the package for any visible damage and ensure all components, including the flow cell and interface cables, are included. Wear gloves and use grounded equipment to prevent static discharge. Place the detector on a stable, level surface and connect the power cord. Install the flow cell as described in the manual, ensuring proper alignment and sealing to prevent leaks. Initialize the detector by powering it on and allowing it to stabilize. Complete any necessary firmware updates and perform a system check to ensure all components are functioning correctly before proceeding to software setup.
4.2. Flow Cell Installation and Connections
Installing the flow cell in the Agilent 1260 Infinity II FLD requires precise alignment to ensure optimal performance. Begin by removing the protective cover from the flow cell and carefully align it with the detector’s flow cell compartment. Secure the flow cell using the provided screws, ensuring no over-tightening to avoid damage. Next, connect the inlet and outlet tubing according to the manual, ensuring proper routing to prevent leaks or blockages. Use compatible tubing materials, such as PEEK, PTFE, or fused silica, as specified. After installation, verify all connections for tightness and perform a system check to confirm proper flow and detector functionality. Always refer to the manual for specific torque and alignment guidelines to maintain warranty and performance integrity. Proper installation ensures accurate and reliable results in fluorescence detection.
4.3. Software and System Setup Requirements
Setting up the Agilent 1260 Infinity II FLD requires compatible software, including Agilent OpenLAB or ChemStation, to control and analyze data; Ensure the system operates on Windows 10 or later, with updated drivers and necessary licenses pre-installed. Connect the detector to the control module via LAN or USB, following the manual’s network configuration guidelines. Verify proper communication by running diagnostic tests within the software. Regularly update software to access new features and maintain system performance. Ensure all system components, such as pumps and autosamplers, are properly integrated and synchronized. Refer to the manual for specific software versions and system requirements to ensure compatibility and optimal functionality. Proper setup ensures seamless operation and accurate fluorescence detection.
Maintenance and Troubleshooting
Regular maintenance ensures optimal performance and extends system longevity. Troubleshooting guides help resolve common issues, while diagnostic tools facilitate quick identification and resolution of detector-related problems.
5.1. Regular Maintenance Procedures
Regular maintenance is essential to ensure optimal performance and longevity of the Agilent 1260 Infinity II FLD. Cleaning the module and flow cell is recommended to prevent contamination and ensure accurate detection. Users should inspect and replace worn or damaged parts, such as seals or tubing, to maintain system integrity. Additionally, checking the flow cell for proper installation and alignment is crucial for consistent results. Routine diagnostic checks using the instrument’s software can help identify potential issues early. Proper handling of lithium batteries and solvents is also emphasized to ensure safety. Following these procedures prevents downtime, enhances sensitivity, and maintains the detector’s reliability for fluorescence detection.
5.2. Replacing the Interface Board
To replace the interface board on the Agilent 1260 Infinity II FLD, start by powering down the detector to prevent any electrical hazards. Ground yourself to avoid static discharge, which could damage the new board. Locate the interface board, typically accessible by removing the detector’s cover. Use a screwdriver to remove the screws securing the board. Carefully pull out the old board and slide in the new one, ensuring proper alignment. Replace the screws and tighten them securely. Finally, power up the detector and run diagnostic tests to ensure proper functionality. Always handle electronic components by the edges and keep the area clean to prevent contamination.
5.3. Cleaning the Module and Flow Cell
Cleaning the Agilent 1260 Infinity II FLD module and flow cell is essential for maintaining optimal performance and preventing contamination. Use a mild detergent and a damp cloth to wipe the module’s exterior, avoiding harsh chemicals or abrasive materials. For the flow cell, flush it with high-purity methanol or distilled water to remove residue. Disconnect the flow cell from the detector, rinse it thoroughly, and allow it to air dry. Regular cleaning ensures accurate fluorescence detection and extends the lifespan of the components. Always refer to the manual for specific cleaning solutions and avoid using organic solvents that may damage the flow cell. Proper maintenance guarantees reliable results and minimizes downtime for your laboratory workflows.
5.4. Common Issues and Diagnostic Features
The Agilent 1260 Infinity II FLD may encounter issues such as low sensitivity, lamp failure, or flow cell blockage. These issues often arise from sources like aged lamps, incorrect excitation/emission settings, or sample contamination. The detector features advanced diagnostic tools, including alarm codes and software alerts, to identify and address problems promptly. Users can utilize the system’s self-test function to evaluate performance and detect anomalies. Additionally, the manual provides troubleshooting guides to resolve common errors, ensuring minimal downtime. By leveraging these diagnostic features, operators can quickly identify and rectify issues, maintaining optimal detector performance and data accuracy in their laboratory workflows. Regular maintenance and proper handling of components further prevent recurring problems.
Safety Information and Regulations
Compliance with WEEE Directive, proper handling of lithium batteries, and UV radiation precautions are essential. Follow safety guidelines for waste disposal and electrical equipment.
6.1. General Safety Precautions
Always follow safety guidelines when handling the Agilent 1260 Infinity II FLD. Wear protective gear, avoid direct UV exposure, and ensure proper grounding to prevent electrical hazards. Handle lithium batteries with care, and dispose of hazardous materials according to local regulations. Regularly inspect cables and connections to prevent damage. Keep the detector away from flammable materials and maintain a clean workspace. Adhere to Agilent’s safety manual for optimal operation and compliance with international standards. Properly label and store chemicals, and ensure ventilation in the laboratory. Follow shutdown procedures to avoid unexpected start-ups. Refer to the manual for detailed safety protocols and emergency procedures.
6.2. Waste Electrical and Electronic Equipment (WEEE) Directive
The Agilent 1260 Infinity II FLD complies with the WEEE Directive (2002/96/EC), ensuring safe disposal of electrical and electronic waste. Users must separate WEEE from other waste and recycle it according to local regulations. Proper disposal prevents hazardous substances from harming the environment. Agilent encourages responsible recycling to minimize ecological impact. Always check local guidelines for WEEE disposal options. By adhering to this directive, users contribute to sustainable waste management and environmental protection. Agilent supports eco-friendly practices throughout the product lifecycle, aligning with global sustainability goals.
6.3. Handling of Lithium Batteries and Solvents
Proper handling of lithium batteries and solvents is crucial for safety and environmental protection. Lithium batteries in the Agilent 1260 Infinity II FLD must be disposed of separately from regular waste, following local regulations to prevent environmental contamination. Solvents used in HPLC systems, such as those compatible with PEEK, fused silica, and PTFE components, should be handled with care to avoid skin contact and inhalation. Always use protective equipment and ensure good ventilation when working with solvents. Dispose of solvents according to local hazardous waste guidelines to minimize ecological impact. Agilent recommends adhering to safety data sheets (SDS) for specific solvent handling instructions. Proper disposal and handling practices safeguard both personnel and the environment, aligning with Agilent’s commitment to safety and sustainability.
6.4. Radio Interference and UV Radiation Safety
The Agilent 1260 Infinity II FLD is designed to minimize radio interference, ensuring compliant operation under global standards. To maintain signal integrity, avoid placing the detector near strong radio frequency sources. Additionally, the system incorporates features to suppress electromagnetic interference, safeguarding data accuracy. For models equipped with UV lamps, proper precautions are essential to prevent exposure to UV radiation, which can cause eye and skin damage. Always wear appropriate protective eyewear when servicing UV components and ensure the lamp is switched off before any maintenance. Adhering to these guidelines ensures safe operation and compliance with safety regulations, protecting both users and the instrument from potential hazards while maintaining optimal performance levels in laboratory settings.
Performance and Applications
The Agilent 1260 Infinity II FLD delivers exceptional sensitivity and selectivity, making it ideal for trace-level component analysis. Its high performance ensures precise detection across various applications, including biochemistry and environmental monitoring. The detector’s linearity and limit of detection (LOD) provide reliable quantification, while its versatility supports diverse chromatographic methods. Whether analyzing fluorescent compounds or complex biological samples, the FLD excels in accuracy and reproducibility, meeting the demands of modern laboratory workflows. Its advanced features and robust design make it a trusted tool for researchers and analysts seeking high-quality results in their applications.
7.1. Sensitivity and Selectivity in Fluorescence Detection
The Agilent 1260 Infinity II FLD offers outstanding sensitivity and selectivity, enabling precise detection of fluorescent compounds at trace levels. Its advanced optical design minimizes light scattering and background noise, ensuring superior signal-to-noise ratio. Selectivity is enhanced through programmable excitation and emission wavelengths, allowing specific targeting of analytes. This reduces interference from non-fluorescent substances, improving accuracy in complex samples. The detector’s high sensitivity is particularly advantageous in biochemistry and pharmaceutical analysis, where low-abundance molecules must be detected. With its robust performance, the Agilent FLD is ideal for applications requiring high analytical precision, delivering reliable results across a wide range of laboratory settings and experimental conditions.
7.2. Linearity and Limit of Detection (LOD)
The Agilent 1260 Infinity II FLD exhibits excellent linearity, with a correlation factor of 0.99998 to 0.99999 for well-resolved amino acids across a range of 10 pmol to 1000 pmol. The limit of detection (LOD) is exceptionally low, typically below 100 fmol, making it suitable for trace-level analysis. This high sensitivity ensures precise quantification of fluorescent compounds, even in complex samples. The detector’s linearity and LOD are critical for applications requiring accurate detection and quantitation of analytes at minimal concentrations. These performance metrics underscore the Agilent FLD’s reliability in delivering high-quality results for demanding laboratory applications.
7.3. Recommended Usage and Applications
The Agilent 1260 Infinity II FLD is ideal for high-sensitivity fluorescence detection in various laboratory settings. It excels in applications requiring trace-level analysis, such as environmental monitoring, pharmaceutical research, and biotechnology. The detector is particularly suited for detecting fluorescent compounds in HPLC workflows, including amino acid analysis, protein studies, and drug development. Its versatility makes it a valuable tool for both routine and complex analytical tasks. With its high selectivity and sensitivity, the Agilent FLD is recommended for applications where precise quantification of low-concentration analytes is critical. It is widely used in industries like pharmaceuticals, biotech, and environmental science for accurate and reliable results.
Appendices and Additional Resources
This section provides additional resources, including a glossary of terms, Agilent Technologies contact information, and links to online user guides and troubleshooting documentation.
8.1. Glossary of Terms and Abbreviations
This section defines key terms and abbreviations used throughout the manual to ensure clarity and understanding. FLD stands for Fluorescence Detector, a device detecting fluorescent signals. LOD refers to Limit of Detection, the smallest detectable amount. DAD is Diode Array Detector, used for UV-Vis detection. UV-Radiation involves ultraviolet light used in fluorescence excitation. WEEE Directive pertains to safe disposal of electronic waste. Glossary provides definitions for technical terms like luminescence (light emission) and excitation (energy absorption). Understanding these terms is essential for effective use of the Agilent 1260 Infinity II FLD.
8.2. Agilent Technologies Contact Information
For inquiries, support, or additional resources, contact Agilent Technologies through their official website: www.agilent.com. Phone support is available at +1-800-227-9770 (US) or +44-208-964-7740 (Europe). Email inquiries can be directed to agilent_support@agilent.com. Regional offices worldwide provide local assistance, ensuring prompt service. Visit the website for specific contact details tailored to your location. Agilent’s customer support team is available to address technical questions, troubleshooting, and ordering inquiries related to the 1260 Infinity II FLD. This section ensures users have direct access to Agilent’s expertise and resources for optimal instrument performance and maintenance.
8.3. Online Resources and User Guides
Agilent Technologies offers extensive online resources and user guides to support the 1260 Infinity II FLD. Visit the official Agilent website at www.agilent.com for access to detailed product manuals, troubleshooting guides, and software updates. Additional resources include instructional videos, technical notes, and application-specific guides. Users can also access the Agilent Community Forum for peer-to-peer support and knowledge sharing. For the 1260 Infinity II FLD, download the G1321-90010 manual directly from the Agilent website. Online training modules are available to enhance operator proficiency. These resources ensure users maximize the capabilities of their Agilent FLD while maintaining optimal performance and compliance with operational standards.